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Research Article - (2017) Volume 9, Issue 4

Antioxidant and Postprandial Glucose-lowering Potential of the Hydroethanolic Extract of Nypa fruticans Seed Mesocarp

Fonkoua Martin1, Nguimkeng Signing Boris1, Sandeu Ruth Kengne1, Tang Eramus Chia1, Takuissu Nguemto Guy1, Azantsa Kingue Boris Gabin1, J. L. Ngondi1* and Gouado Innocent2
1Department of Biochemistry, Faculty of Science, University of Yaounde I, Yaounde, Cameroon
2Department of Biochemistry, Faculty of Science, University of Douala, Douala, Cameroon
*Corresponding Author: J. L. Ngondi, Department of Biochemistry, Faculty of Science, University of Yaounde I, Yaounde, Cameroon Email:


Postprandial hyperglycemia and oxidative stress are implicated in the pathophysiology of type 2 diabetes. An alternative treatment should target both conditions. The present study aimed to evaluate the antioxidant and postprandial glucose-lowering potential of a polyphenolic rich extract of Nypa fruticans nuts. Hydroethanolic extract (HEE) was prepared; polyphenol and fl avonoid content was determined. In vitro antioxidant activity was done through molybdenum (VI) reduction and ABTS and DPPH radical-scavenging capacities. The glucose-lowering potential of the extract was evaluated through in vitro glucose-binding capacity, the regulatory ability of the extract on the pancreas of normal Wistar rats (oral glucose tolerance test), and digestion of carbohydrates (starch and sucrose) by normal Wistar rats. Starch (1 g/kg of the body weight (BW) and sucrose (2 g/kg BW) solution were used as substrates for alpha amylase and invertase inhibition test, respectively. Acarbose (3 mg/kg BW) was used as the reference. Polyphenolic-rich extract (400 mg/kg BW) was administered to treated rats. Results showed that HEE of N. fruticans nuts has antioxidant activity through the scavenging of ABTS (EC50 of 1.75 mg/mL) and DPPH (2.96 mg/mL) radicals and the reduction of Mo (VI). The extract signifi cantly reduced glucose concentrations at t30 compared to glibenclamide, but glibenclamide maintained lower glycemia than the extract thereafter to the end of the study. From the administration of sucrose, the extract maintained lower plasma glucose levels compared to acarbose throughout the study. However, no inhibition was observed in glycemia following starch administration in HEE-treated group as glycemia remained higher compared to the acarbose group throughout the study. Thus, N. fruticans seed mesocarp is a rich source of dietary polyphenols with a strong antioxidant capacity. Its HEE has glucose-lowering potential and possible inhibitory action on the last phase of carbohydrate digestion.

Keywords: Postprandial hyperglycemia, Glucose-binding potential, Glycemic reduction, Nypa fruticans mesocarp


Diabetes is a metabolic disorder characterized by chronic hyperglycemia. Extended postprandial hyperglycemia and high fasting plasma glucose levels are the major determinants of diabetes. High fasting plasma glucose is caused by increased hepatic release of glucose and the inability of peripheral cells to absorb the glucose, linked to insulin defi ciency and insensitivity. Th is results in disturbances in carbohydrate, fat, and protein metabolism. Postprandial hyperglycemia results from the persistent and extended increase in plasma glucose and depends on the digestibility of carbohydrates and the regulatory capacity of pancreatic cells. Postprandial hyperglycemia leads to protein glycation and glucose oxidation, releasing oxygen and nitrogen reactive species. Oxidative stress is known to play a signifi cant role in the development and progression of diabetes mellitus (DM). Free radicals from oxygen (reactive oxygen species (ROS)) and nitrogen (reactive nitrogen species (RNS)) are produced in the internal membrane of the mitochondria, in the endothelial vascular cells, and in the endoplasmic reticulum during auto-oxidation involving physiological molecules like NADH, NADPH, and neurotransmitters. ROS at low or moderate concentrations in human tissues are required for optimum cellular functioning but when produced in excess, they lead to cell and tissue injury. Th e damage caused by surplus free radicals in tissues is referred to as oxidative stress. Oxidative stress aggravates type 2 DM [1]. Th e incidence and prevalence of diabetes is on a global rise and is alarming in sub-Saharan Africa and Southeast Asia [2] (Wild et al., 2004), which are resource-scarce regions of the world. To face this pandemic, promising control strategies target diet, lifestyle, and carbohydrate metabolism. Diet and lifestyle modifi cations are considered the cornerstone for the treatment and management of type 2 diabetes. In addition to diet and lifestyle, pharmaceutical molecules including sulfonylureas (which stimulate insulin release) and carbohydrate enzyme inhibitors, like acarbose and miglitol, are used in monitoring type–2 DM. Th ese drugs mainly aim at reducing postprandial upsurge in blood glucose level or delaying glucose release into the bloodstream, thereby modulating glycemia. Evidence holds that the way forward for a better monitoring of DM should target natural carbohydrate inhibitors [3] and natural antioxidants [4], which present little or no deleterious eff ects compared to the synthetic pharmaceutical molecules. Many plant antioxidants have hypoglycemic eff ects, which may also contribute to improved glycemia. Plant foods (fruits, spices, and vegetables) are important dietary sources of antioxidant polyphenols to humans. Several plant preparations are traditionally used in Cameroon to treat diabetes. Some of them have been screened for bioactivity of crude extract, but only few studies report on Nypa fruticans seed mesocarps. N. fruticans is one of such plants.

N. fruticans is a tropical mangrove plant of the family of Palmae/ Arecaceae whose leaves, stems, and roots have been traditionally used in the treatment of asthma, leprosy, tuberculosis, sore throat, liver disease, and snake bite, as a sedative and carminative [5]. Th e fruit is also rich in carbohydrates, fi bers, minerals, and vitamin A [6]. Th e hexane, ethyl acetate, and methanolic extract of its leaves and fruits showed in vitro antioxidant activity [7], while the methanolic extract of its leaves and stem showed antihyperglycemic activity in mice [8]. This work seeks to investigate the in vitro and in vivo potency of the hydroethanolic extract (HEE) of N. fruticans seed mesocarp to lower blood glucose level.

Materials and Methods

Chemical reagents

All chemicals used were of analytical grade. Folin-Ciocalteu reagent, gallic acid, AlCl3, potassium acetate, cyanidic acid, ethanol, vanillin, hydrochloric acid, 1,1-diphenyl-2-picrylhydrazyl (DPPH), 2,2′-azinobis-(3-ethylbenzothiazoline 6-sulfonic acid) (ABTS), KMnO4, phosphate buffer, sulfuric acid, sodium phosphate, ammonium molybdate, glucose, glucose test kit, starch, and sucrose were purchased from SIGMA (USA).

Plant collection and preparation of extracts

Fresh N. fruticans fruits were harvested in the Littoral Region of Cameroon in January 2015 and together with the stems and bark of the tree were authenticated at the National Herbarium in Yaounde (Voucher No38371/HNC). The fruits were washed with running water. The outer layer was removed and the seed mesocarp recovered for subsequent use. The mesocarps were then air-dried in the shade (room temperature) for five days and the dried matter was ground to powder using a small scale grinding machine.

Preparation of the HEE of N. fruticans mesocarp

To get the polyphenol rich extract, 500 g of N. fruticans mesocarp powder was macerated in 4000 mL (1/8) of water and 95% ethanol mixture (1:1). The filtrate was evaporated at 50C in an oven and the yield for extraction was calculated (10%).

Determination of polyphenol and flavonoid content

Polyphenol content

Polyphenol content was evaluated on the basis of the residue’s capacity to reduce phosphomolybdic phosphor-tungstic acid (Folin- Ciocalteu) reagent to a blue-colored complex in an alkaline solution [9]. The measurement was done using the Folin-Ciocalteu reagent (Sigma Chemical Co. St. Louis, MO) diluted 10 times before use. Briefly, 30 μL of 1 mg/mL of the extract and 1 mL of diluted Folin-Ciocalteu reagent were pipetted into a tube and incubated for exactly 30 min. Parallel to the Nypa extract tubes were the 30-μL catechin standard tube and the blank tube containing 30 μL of distilled water. Absorbance was read at 750 nm in a UV spectrophotometer. Total polyphenol was expressed in milligram equivalents of gallic acid per gram of dry mesocarp powder (mg EG/g DM).

Total flavonoids

Total flavonoid content was evaluated using the method described by Aiyegoro and Okoh [10]. The method is based on the fact that flavonoids react with aluminum chloride and potassium acetate to form a pink solution whose absorbance can be measured at a wavelength of 420 nm. Briefly, 1 mL of the extract (1 mg/mL) was mixed with 3 mL of ethanol (95%), 0.2 mL of 10% aluminum chloride, 0.2 mL of 1 M potassium acetate, and 5.6 mL of distilled water and incubated at room temperature for 30 min. Thereafter, the absorbance was directly measured at 420 nm with UV visible spectrophotometer. Flavonoid content was then determined from the calibration curve prepared from catechin solution (0-1000 μg/mL) in distilled water. Flavonoid content was expressed in milligram equivalents of gallic acid per gram of dry mesocarp powder (mg EG/g DM).

Determination of the antioxidant capacity

Total antioxidant capacity

Total antioxidant capacity was measured using the method described by Prieto et al. [11]. This technique is based on the reduction of molybdenum (Mo VI) to Mo (V) by the test sample and the subsequent formation of a green phosphate/Mo (V) complex in acid solution with optimum absorbance at 695 nm. A volume of 0.3 mL of extract (5 mg/mL) was mixed with 3 mL of the reagent (0.6 M sulfuric acid, 28 mM sodium phosphate, and 4 mM ammonium molybdate). All the tubes were sealed and incubated at 95C for 90 min. After cooling the tubes, the absorbance of the solutions was measured at 695 nm against the blank containing 3 mL of the reagent and 0.3 mL of methanol. The total antioxidant capacity was expressed in milligram equivalents of ascorbic acid per gram of dry mesocarp powder (mg EA/g DM).

Free radical-scavenging activity

The scavenging activities of the free radicals were evaluated using two synthetic radicals: 2,2-azinobis (3-ethyl-benzothiazoline-6- sulfonic acid) (ABTS1) for lipophilic and hydrophilic antioxidants and 1,1-diphenyl-2-picrylhydrazyl (DPPH•) for lipophilic antioxidants.

The DPPH radical-scavenging activity was measured according to the procedure reported by Katalinie et al. [12]. This method relies on the decrease in absorbance measured at 515 nm, based on the capacity of the antioxidant to trap free radicals forming a stable DPPH-H. Volumes of 50 μL of extracts (2, 4, 6, 8 and 10 mg/mL) were introduced in 1.950 mL of an ethanol solution of DPPH. After 30 min of incubation in the dark, the absorbance was measured at 515 nm against the blank containing the reagent.

The ABTS radical-scavenging activity was measured following a modified procedure reported by Re et al. [13]. In the presence of potassium permanganate (KMnO4), blue ABTS is oxidized to green ABTS1 radical. Antioxidants reduce green ABTS1 radical with discoloration of the solution. Discoloration capacity measured at 734 nm is directly proportional to the antioxidant concentration. The extracts were prepared at different concentrations (2, 4, 6, 8, and 10 mg/mL) and 1 μL of each concentration was put in a tube containing 1000 μL of ABTS1 and incubated for 30 min in the dark. The absorbance was read against the blank containing the reagent. The percentage inhibition of the free radical by the polyphenolic rich extract was calculated using the formula below:


The half efficient concentration (EC50), which reflects a 50% decrease of DPPH or ABTS radical by the extract, was derived and expressed in mg/mL. EC50 was determined using the percentage scavenging capacity of all the concentrations at the stationary stage followed by the application of logarithmic regression analysis.

Evaluation of the in vitro hypoglycemic activity of the extract

Glucose-binding capacity was evaluated through the glycosylation test described by Kebieche [14]. This test is based on the ability of extracts to form complexes with free glucose, demonstrating a glucophage activity. A standard glucose solution was prepared (10 mM), from which 100 μL was pipetted into seven tubes and mixed with 100 μL of the polyphenol-rich extract of N. fruticans seed mesocarp powder at different concentrations (0.5, 1, 2, 4, 8, and 16 mg/mL). The mixture obtained was gently homogenized and incubated at 37°C for 15 min. Glucose concentration was then measured using the SGM Italia kit as described by Trinder [15] and the absorbance read at 505 nm. A control was made in absence of the extract. The percentage of glucose binding was obtained using the following formula:


Postprandial glucose-lowering potential in normoglycemic rats

Adult male albino Wistar rats weighing 180-200 g were obtained from the Department of Zoology of the University of Yaounde I. They were housed in clean cages with five rats per cage and maintained under standard laboratory conditions (room temperature with dark/light cycle 12/12 h) for three days and fed with a standard diet and normal tap water.

Oral glucose tolerance test on normoglycemic rats

Oral glucose tolerance test (OGTT) was performed on thirty (30) male albino Wistar rats. The rats were acclimated for 7 days and divided into six groups with each group consisting of five rats. The rats were fasted overnight for 18 h but had access to water and their glycemia was taken at the start of the test (t0). Half an hour before the administration of the standard glucose solution (2 g/kg of body weight (BW), each group of rats received either water, a hypoglycemic drug (glibenclamide 4 mg/kg BW), or the extract (400 mg/kg BW), except for the negative control group, which received water. The treatment lasted for 2 h and their glycemia was measured at 30-min time intervals (30, 60, 90, 120 min) using a glucometer [15].

Inhibitory effects of extracts on starch and sucrose digestion in normoglycemic rats

The OGTT procedure described above with slight modifications was used. Modifications included 2 g/kg BW of sucrose and 1 g/kg BW of starch, used to replace glucose. Acarbose (3 mg/kg BW) was used as the reference drug. Blood glucose was measured at 30, 60, 90, and 120 min.

Statistical analysis

Results were expressed as mean ± standard deviation. Statistical analyses were done using the Statistical Package for Social Science (SPSS) software, version 17.0, for Windows. One-way analysis of variance (ANOVA) between groups was done with LSD (least significant difference) as post hoc test to compare the in vivo results. Significant differences were detected at 95% confidence interval.


Polyphenolic compounds and antioxidant activities of HEE of N. fruticans seed

The polyphenolic compounds and total antioxidant capacity of the Nypa seed mesocarp extracts are shown Table 1. Total polyphenol content was 42.87 mg EG/g DM and flavonoid content 19 μg EG/g DM. The total antioxidant capacity of the extract (5 mg/mL) was 0.47 mg EAA/g DM.

  Total polyphenols (mg EG/g of DM) Flavonoids (µg EG/g of DM) Total antioxidant capacity (mg EA/g of DM)
of N. fruticans
42.87 ± 0.76 19 ± 0.5 0.47 ± 0.01

DM: dry mesocarp and the result as mean ± standard deviation; EA: equivalent ascorbic acid; EG: equivalent gallic acid.

Table 1: Total polyphenol and flavonoid contents and total antioxidant capacity of HEE of N. fruticans seed

DPPH and ABTS1 free radical-scavenging ability were examined at five different concentrations (2, 4, 6, 8, and 10 mg/mL) and the results expressed as scavenging activity is presented in Figure 1. All extract concentrations expressed strong scavenging capacity, which increased with concentration, ranging from 42.13% to 72.50% for DPPH and from 49.34% to 89.90% for ABTS. Half scavenging concentrations (SC50), derived through logarithmic regression analysis from the graph, were found to be 2.96 mg/mL for DPPH and 2.21 mg/mL for ABTS. Thus, the extract scavenging capacity of ABTS1 cation was stronger than that of the DPPH free radical.


Figure 1: DPPH- and ABTS-scavenging activities of HEE of N. fruticans seed

Postprandial glucose-lowering activities of HEE of N. fruticans seed in vitro assay

Glucose-binding capacity expressed as the percentage of glucose reduction of the extract is shown in Figure 2. Glucose binding was found to increase with extract concentration and ranged from 9.19 (0.5 mg/mL) to 57.17% for (16 mg/mL).


Figure 2: Glucose-binding capacity of HEE of N. fruticans seed

Effect of HEE on glucose absorption

The ability of the extract to regulate postprandial hyperglycemia following oral administration in normoglycemic rats is presented in Table 2. Administration after 30 min (t30) of glucose loading (10 mM) in the test groups resulted in glycemia peak in the positive group higher than the peak obtained with the negative group throughout the experiment. In the group receiving extracts, glycemia (68.60 mg/ mL) at 30 min after glucose loading was lower than that of the positive control group (103.71 mg/mL). However, glycemia considerably reduced to the same level in the two groups at the 180th min (59.80 and 59.85 mg/mL, respectively). The extract group also expressed a lower glycemic levels at t30 compared to the reference (glibenclamide) group (90.8 mg/mL). Thereafter, glycemia significantly reduced to the 180th min in the reference group (20 mg/mL) compared to the extract group.

Time groups t0 t30 t60 t90 t120
NC 46.20 ± 10.90a
50.40 ± 6.69a
52.80 ± 7.11a
38.80 ± 6.40a (−16.01) 40.00 ± 3.01a (−13.41)
PC 46.57 ± 6.36a
103.71 ± 5.26b (122.69) 87.28 ± 4.47b
71.71 ± 9.27b
59.85 ± 7.15b
N fru 46.20 ± 14.00a
68.60 ± 4.17a
72.80 ± 6.14b
81.80 ± 7.39b
59.80 ± 3.34b
REF 45.40 ± 8.32a
90.80 ± 6.92c
40.60 ± 9.68a (−10.57) 22.60 ± 0.97a (−50.22) 25.00 ± 2.68a (−44.93)

NC: negative control; PC: positive control; REF: reference; and N fru: N. fruticans. The values in parentheses are the percentage variation; results are expressed as mean in glycemia ± standard error of mean. Values having differences indices had significant differences at 5%.

Table 2: Effect of HEE of N. fruticans seed on glucose tolerance

Effect of HEE on starch digestion and absorption

The variation in glycemia following the administration of starch digestion is presented in Table 3. The positive control expressed the highest glycemic values compared to the negative group throughout the experiment. At 30 min, glycemia was highest in the group receiving extract (95.4 mg/mL) compared to the positive control (76.14 mg/mL) and the reference (acarbose) (75.6 mg/mL) groups. However, at t180, glycemic variation had reduced in all the groups below the initial glycemic state.

Groups t0 t30 t60 t180
NC 66.40 ± 6.22a
58.80 ± 11.04a (−11.44) 58.00 ± 12.11a (−12.65) 51.20 ± 10.24a (−22.89)
PC 59.71 ± 8.15a
76.14 ± 7.16b
63.85 ± 4.41b
54.00 ± 3.80ab (−9.56)
N fru 67.80 ± 6.21a
95.40 ± 9.47c
78.00 ± 3.17c
61.60 ± 2.59bc (−9.14)
REF 66.20 ± 7.27a
75.60 ± 11.64b
77.00 ± 13.84c
64.40 ± 3.69c (−2.71)

NC: negative control; PC: positive control; REF: Reference; and N fru: N. fruticans. The values in parentheses are the percentage variation; results are expressed as mean in glycemia ± standard error of mean. Values having differences indices had significant differences at 5%.

Table 3: Effect of HEE of N. fruticans seed on starch digestion

Effect of HEE on sucrose digestion and absorption

Glycemic variation from sucrose digestion in rats following the oral administration of the extract and control preparations is presented in Table 4. The positive control group had the highest postprandial glucose level compared to the negative control group throughout the study. The group receiving extract also had the lower plasma glucose compared to the positive group throughout the study period. In the same way, the extract group expressed lower plasma glucose level compared to the reference group throughout the study. The glycemia was 73.58 mg/mL for the extract group against 90.14 and 82.80 mg/mL for the positive and extract groups, respectively.

Groups t0 t30 t60 t180
NC 66.40 ± 6.22a
58.80 ± 11.04a (−11.44) 58.00 ± 12.11a (−12.65) 51.20 ± 10.24a (−22.89)
PC 59.71 ± 8.15a
76.14 ± 7.16b
63.85 ± 4.41b
54.00 ± 3.80a,b (−9.56)
N fru 67.80 ± 6.21a
95.40 ± 9.47c
78.00 ± 3.17c
61.60 ± 2.59b,c (−9.14)
REF 66.20 ± 7.27a
75.60 ± 11.64b
77.00 ± 13.84c
64.40 ± 3.69c (−2.71)

NC: negative control; PC: positive control; REF: Reference, and N fru: N. fruticans. The values in parentheses are the percentage variation and the results expressed as mean in glycemia ± standard error of mean. Values having differences indices had significant differences at 5%.

Table 4: Effect of HEE of N. fruticans seed on sucrose digestion


One of the major problems in the management of DM is the control of postprandial hyperglycemia, which depends on digestion and absorption of carbohydrates and stimulation of incretins [16], which increase the release of insulin by beta-pancreatic cells. Hyperglycemia is highly correlated to the increase of pro-oxidants like free radicals, which cause oxidative stress, proven to be implicated in the development and complication of diabetes. Screening of bioactive compounds, which can act both on postprandial glycemia and on oxidative stress, could be a better alternative in the management of diabetes. This study revealed that HEE of N. fruticans seed mesocarp possesses high total polyphenol content and total antioxidant capacity in a concentration-dependent manner (Table 1). These results are indicators of a possible antioxidant capacity of HEE and demonstrate their ability to reduce Mo (VI) to Mo (V). Phenolic compounds are secondary metabolites reported to exert physiological effects [17]. These results in accordance with many other studies [18], could also be explained by the fact that related polyphenols and many flavonoids contribute significantly to the phosphomolybdenum reduction activity through the transfer of electrons as showed by Khan et al. [19].

An exposure of HEE with nonbiologically generated free radicals DPPH and ABTS, which are synthetic radicals commonly used to evaluate the scavenging activity of plant extract, has shown a scavenging activity on DPPH and ABTS radicals (Figure 1). This result is in accordance with those of Nagendra et al. [20], who showed that the extracts of ripe and unripe endosperm of N. fruticans possess the DPPHand ABTS-scavenging abilities. The DPPH radical has been widely used to evaluate free radical-scavenging capacity of antioxidants. It contains an odd electron, which is responsible for the violet color. When DPPH accepts an electron donated by an antioxidant compound, the DPPH is decolorized to yellow [21]. Also, the blue/green ABTS radical cation is generated by the oxidation of ABTS with potassium persulfate; its reduction in the presence of hydrogen-donating antioxidants is measured spectrophotometrically at 745 nm. The scavenging effects observed can be attributed to the presence of flavonoids in HEE extract, due to their redox properties. Flavonoids play an important role in absorbing and neutralizing free radicals, by donating hydrogens or electrons [22].

Phenolic compounds also exhibit strong hypoglycemic effects through several mechanisms including inhibition of carbohydrate digestive enzymes, regulation of pancreatic function, and glycosylation [23]. The in vitro and in vivo studies on the modulatory effects of hydroethanolic extract on glucose absorption and carbohydrate digestion revealed that the extract possesses the ability to scavenge glucose (Figure 2) through glycosylation, although such glycosylation may instead improve other therapeutic actions of the extract [24]. In fact, phenolic compounds, such as flavonoids, found in HEE can directly react with glucose molecules to form a flavonoid-glucose complex, thereby reducing the amount of free glucose. The glycosylated glucose molecule therefore would be unable to cross cell membranes, hence the reduction of glucose absorption due to conformational structures and chemical and physical changes affecting biochemical functions [24]. Given that in vitro studies only show the direct interaction between extract and substrate, the postprandial glucose-lowering potential of the of the N. fruticans seed mesocarp was done under physiological conditions so that many other factors could be involved. The oral glucose tolerance test conducted to evaluate the effects of HEE on pancreatic beta-cells function revealed their lowering potential on postprandial glycemia after glucose and sucrose administration (Tables 2 and 4). This result is in accordance with the findings of Wang et al. [25] and Hasan et al. [8] and can be attributed to polyphenols, which may suppress glucose release from the liver and improve glucose uptake in peripheral tissues by modulating intracellular signaling [23]. Hasan et al. [8] also demonstrated that methanolic leaves and stem extracts of N. fruticans decrease glycemia at 60 and 120 min after glucose loading. Similar results were also found by Yusoff et al. [26] on the aqueous extract of N. fruticans Wurmb after glucose and sucrose or starch loading. However, the hydroethanolic extract of N. fruticans seed mesocarp showed an increase of postprandial glycemia after starch administration (Table 3); it may be due to the high content of carbohydrates present in the seed mesocarp [6], proving that HEE probably has no inhibitory effect on amylase activity. Several mechanisms could explain the results observed and the hyperglycemia activity of the hydroethanolic extract of N. fruticans nuts. These include inhibition of sucrose activity, inhibition of intestinal glucose absorption, stimulation of insulin secretion by beta pancreatic cells, and stimulation of glucose utilization [18].

Flavonoids or phenolic compounds have been associated with inhibition of alpha-glucosidase [27]. The hydroethanolic extract of N. fruticans could be ameliorating postprandial hyperglycemia by competing with glucose for the binding site on glucose transporters, thus delaying the process of glucose absorption. However, it is important to note that if the inhibition mechanism could be noncompetitive, this could prevent the presence or absence of substrate from affecting the binding of the inhibitor to the enzyme, resulting in less interaction with meals [28,29]. Hassimotto et al. [30] reported that one of the intestinal glucose uptake mechanisms influenced by a flavonoids probably involved SGLT1. Glycemic decrease observed with HEE may also occur due to the stimulatory effect on beta cells to promote insulin release given that insulin stimulates an intracellular glycogen deposition and glucose utilization [31]. Furthermore, HEE can reduce PPG by stimulating early insulin release after sucrose administration that increases and more reduction of glucagon secretion, resulting in regularization of glucose homeostasis [16,32].


N. fruticans seed mesocarp is a rich source of dietary polyphenols with high antioxidant capacity and radical-scavenging capacity. The hydroethanolic extract of seed mesocarp has in addition a glucophage activity and inhibitory effect on the last phase of carbohydrate digestion. Therefore, consumption of N. fruticans seed mesocarp, already an eating habit in Asia and Africa, should be encouraged and extended to other continents. In combination with other existing dietary measures to manage diabetes, control strategies against postprandial hyperglycemia and its related complications would probably be more efficient.

Conflict of Interests

None declared.

Authors’ Contributions

All authors contributed equally to this study.


Citation: Martin F, Boris NS, Kengne SR, Chia TE, Guy TN, et al. (2017) Antioxidant and Postprandial Glucose-lowering Potential of the Hydroethanolic Extract of Nypa fruticans Seed Mesocarp. Biol Med (Aligarh) 9: 406.

Copyright: © 2017 Martin et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.