Johnson Siallagan

Department of Chemistry, University of Cenderawasih, Papua Province, Indonesia

Publications
  • Research Article   
    Genetic Mutations in the Papuan Human Mitochondrial Genome: Studies in Gene Control Regions and Gene Coding Using REPLI-g
    Author(s): Yohanis Ngili* and Johnson Siallagan

    Comparative studies of analysis and DNA mutations in Papuan humans and its comparison with several world ethnicities, both in the coding region and the gene control region have been conducted. The objective of this research was to analyze mutation variants in all regions of the complete genome of the human mitochondria by using the G-repli technique for amplification of the mtG, the results of nucleotide sequencing were then compared on several individuals representing some ethnicities in the world. DNA samples were isolated from human tissue and then sequenced using efficient primary pairs to amplify human mtG. Here, we report that the results of mutation analysis through the mtDNA cloning process can be concluded that variations in the length of the poly-C series in the same sample indicate the presence of mtDNA subpopulations in certain individuals, also known as heteropl.. View more»

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