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Adenike AOO
Centre for Petroleum, Energy Economics and Law, University of Ibadan, Oyo State, Nigeria
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Review Article
The Intriguing Extrapolations of Haemolysis Assay as Screening Criterion for Selecting Biosurfactant- Producing Microorganisms in Petroleum Industries Process-Conditions
Author(s): Adenike AOO* and Falode OA
Commonly-used screening techniques for determination of biosurfactants production by microorganisms include haemolysis assay, generally depicted to confirm the ability of microorganisms in production of biosurfactants. Diameters of zones of haemolysis surrounding microbial colonies are considered as quantitative indication of biosurfactant production whereas; haemolytic reactions on blood agar plates are specifically associated with pathologic types of erythrocyte lysis by microorganisms, due to haemolysins production. Haemolytic microorganisms can destroy erythrocyte membranes, by compromise in integrity of cytoplasmic membranes, through pore-forming mechanisms, multiple-hit mechanism, formation of sphaerocytes, derangement of membrane integrity, detergentlike action, or lipase activity. Relative levels of acute toxicity, cell invasiveness and virulence fa.. View more»