Journal of Microbial & Biochemical Technology

Yoshihiro Usuda

Publications

• Research Article
  Metabolic Control of the TCA cycle by the YdcI Transcriptional Regulator in Escherichia coli
  Author(s): Yousuke Nishio, Tomoko Suzuki, Kazuhiko Matsui and Yoshihiro UsudaYousuke Nishio, Tomoko Suzuki, Kazuhiko Matsui and Yoshihiro Usuda
  
  Understanding the regulation and control of the expression of genes encoding metabolic enzymes is crucial for production using microbes. To overcome technical difficulties involved in identifying regulatory network systems, we designed a DNA motif finding procedure combining transcriptome and genome sequence data. Here, we used the ArcAB two-component system of Escherichia coli, which controls genes involved in the TCA cycle and energy metabolism, as a model to identify DNA motifs involved in gene-expression regulation. DNA-array data were used to extract up-regulated genes from ΔarcA and ΔarcB E. coli strains, and the upstream sequences were subjected to DNA-motif finding. Sequence similarity and conserved residues identified the known ArcA-binding motif and a novel DNA-motif candidate that was estimated to be related to YdcI, a putative LysR-type transcriptional regulato.. View More»
  DOI: 10.4172/1948-5948.1000101

  Abstract PDF