Journal of Microbial & Biochemical Technology

Yanhong Liu

Publications

• Research Article
  Comparison of Different Methods for Isolation of Bacterial DNA from Retail Oyster Tissues
  Author(s): Qian Zhang, Min Dai, Yanhong Liu, Min Zhou, Xianming Shi and Dapeng WangQian Zhang, Min Dai, Yanhong Liu, Min Zhou, Xianming Shi and Dapeng Wang
  
  Oysters are filter feeders that bioaccumulate bacteria in water while feeding. To evaluate the bacterial genomic DNA extracted from retail oyster tissues, including the gills and digestive glands, four isolation methods were used. Genomic DNA extraction was performed using the Allmag™ Blood Genomic DNA (Allrun, Shanghai, China), the MiniBEST Bacterial Genomic DNA Extraction kits (Takara, Dalian, China), and the phenol-chloroform and boiling lysis methods. The concentration of the genomic DNA was measured using a spectrophotometer. The purity of the genomic DNA was evaluated by PCR amplification of 16S rDNA followed by determining the cloning efficiency of the amplicon into the pMD19-T vector. Furthermore, the bacterial DNA quality was also evaluated by PCR assays using a pair of species-specific primers for Vibrio parahaemolyticus. Our results showed that the two commercial kits.. View More»
  DOI: 10.4172/1948-5948.1000146

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