Biochemistry & Analytical Biochemistry

Wenchao Shan

Publications

• Research Article
  Preparation of a Monoclonal Antibody Against Saikosaponin C and the Establishment of its Enzyme-Linked Immunosorbent Assay
  Author(s): Baoping Qu, Jiayang Sai, Huihua Qu, Shuchen Liu, Jinjun Cheng, Wenchao Shan, Yan Zhao and Qingguo WangBaoping Qu, Jiayang Sai, Huihua Qu, Shuchen Liu, Jinjun Cheng, Wenchao Shan, Yan Zhao and Qingguo Wang
  
  A monoclonal antibody (MAb) 1E11D8 against saikosaponin c (SSc) was prepared by a cell fusion with splenocytes and hypoxanthine-aminopterin-thymidine-sensitive mouse myeloma SP2/0 cell line. The prepared anti-SSc MAb- 1E11D8 has a novel characteristic which shows high specific for saikosaponin c, saikosaponin d and saikosaponin a, three major oleanane-saponins in Radix Bupleuri. By using anti-SSc MAb-1E11D8, a realiable ELISA was developed for the detection of SSc. The system shows a full measuring range from 156.25 ng·mL-1 to 2500 ng·mL-1 in the case of SSc in indirect competitive ELISA (icELISA). The regression equation was y=-0.283 ln(C) +2.3301 with a correlation coefficient of 0.99. Precision and accuracy of the icELISA method were evaluated by the variations between replicates from well to well (intra-assay) and plate to plate (int.. View More»
  DOI: 10.4172/2161-1009.1000196

  Abstract PDF