Pharmaceutica Analytica Acta

Takuhiro Uto

Publications

• Review Article
  Quality Control of Natural Products by Fingerprinting of Eastern Blotting
  Author(s): Shunsuke Fujii, Nguyen Huu Tung, Takuhiro Uto, Hiroyuki Tanaka, Xiao-Wei Li, Shao-Qing Cai, Waraporn Putalum and Yukihiro ShoyamaShunsuke Fujii, Nguyen Huu Tung, Takuhiro Uto, Hiroyuki Tanaka, Xiao-Wei Li, Shao-Qing Cai, Waraporn Putalum and Yukihiro Shoyama
  
  Eastern blotting was developed for small molecule compounds which have no affinity of conjugation to membrane instead of western blotting. Crude extracts of medicinal plants were developed by TLC plate followed to be blotted to polyvinylidene fluoride or polyether sulfone membrane by heating. Components on membrane were conjugated with carrier protein resulting in fixing to membrane. Staining was followed to western blotting system using monoclonal antibody (MAb). Four types of natural products, solasodine glycosides, aristrochic acid, glycyrrhizin-liquiritin and ginsenosides were discussed in this review. 1) Solasodine glycosides are selected as an example of staining for almost all solasodine glycosides using the anti-solamargine MAb having wide cross-reactivity. 2) Aristrochic acids possess no capacity of conjugation with membrane and their aqueous solubility is quite poor. To impr.. View More»
  DOI: 10.4172/2153-2435.1000494

  Abstract PDF