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Surana SJ
Surana SJ
India
Publications
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Research Article
HPTLC and RP-HPTLC Method Development and Validation for the Estimation of Felodipine in Bulk and Pharmaceutical Formulation
Author(s): Jain PS, Ansari NA and Surana SJJain PS, Ansari NA and Surana SJ
The aim of this work is to establish developed and validated method for the pharmaceutical analysis of Felodipine in bulk and pharmaceutical formulation by High Performance Thin Layer Chromatography HPTLC (NP) and Reverse Phase-High Performance Thin Layer Chromatography RP-HPTLC (RP). Chromatographic separation was performed on Pre-coated aluminum plates with 250 μm layer of Silica gel 60 F254 and Silica gel 60 RP-18 TLC F254S using Toluene: Methanol (8:2 v/v) and acetonitrile: water: glacial acetic acid (8:2:1 v/v/v) as a mobile phase, respectively. Scanning was carried out densitometrically at 237 nm. The Rf value of Felodipine in NP and RP were 0.40 and 0.53 and the reliability of the method was assessed by the evaluation of linearity which was found to be 300-1800 and 500-3000 ng/band with the r2 =0.998 correlation coefficient along w.. View More»
DOI: 10.4172/2153-2435.1000599