Journal of Carcinogenesis & Mutagenesis

Shigueko Sonohara

Publications

• Research Article
  Detection of c-MYC Gene in Micronucleated Hepatocytes from Regenerative Cirrhotic Nodules and Hepatocellular Carcinoma of Hepatitis C Virus Infected Patients
  Author(s): Terezinha Morato Bastos de Almeida, Regina Maria Cubero Leitão, Maisa Yoshimoto, Joyce Anderson Duffles Andrade, Willy Beçak, Flair José Carrilho and Shigueko SonoharaTerezinha Morato Bastos de Almeida, Regina Maria Cubero Leitão, Maisa Yoshimoto, Joyce Anderson Duffles Andrade, Willy Beçak, Flair José Carrilho and Shigueko Sonohara
  
  c-MYC gene alteration has already been shown in chronic liver diseases, which includes hepatocellular carcinoma (HCC). The spontaneous formation of micronucleated hepatocytes (MN-Heps) in liver cirrhosis (LC) tissue from patients infected by hepatitis C virus (HCV), has been previously reported. The objective of this study was to investigate if the c-MYC gene sequence is lost by MN-Heps in cirrhotic process with or without HCC. For this purpose the presence of c-MYC gene in MN-Heps was investigated by fluorescence in situ hybridization in paraffin-embedded liver tissue. Five control liver samples of healthy organ donors were included in this study. Increased c-MYC gene copies were detected in 48-66% of MN-Heps in cirrhotic nodules from all cases, but not in control liver cells. Furthermore, gain of c-MYC gene number of copies was detected i.. View More»
  DOI: 10.4172/2157-2518.1000130

  Abstract PDF