Pharmaceutica Analytica Acta

Shan-Yang Lin

Publications

• Research Article
  Thermal Fourier Transform Infrared Technique Quickly Detects an Early Onset of Glycation-Induced Conformational Changes of Human Serum Albumin
  Author(s): Yu-Ting Huang, Hui-Fen Liao, Shun-Li Wang and Shan-Yang LinYu-Ting Huang, Hui-Fen Liao, Shun-Li Wang and Shan-Yang Lin
  
  Numerous potential inhibitors for prevention of advanced glycation end products (AGEs) formation have been extensively investigated for their in vitro and in vivo features, but it would spend a lot of time studying. In the present study, a unique thermal Fourier Transform Infrared (FTIR) combined system as an accelerated method was attempted to simultaneously determine the thermal-dependent conformational changes of human serum albumin (HSA) in the HSA-ribose mixture and examine the onset of the structural transformation from α-helix to β-sheet structures with or without AGEs inhibitors used. The present results clearly indicate that native HSA had an onset temperature at 96°C for the irreversible thermal-induced structural transition from α-helices to β-sheets, whereas HSA-ribose mixture exhibited its onset temperature near at 78°C due to the early occur.. View More»
  DOI: 10.4172/2153-2435.1000512

  Abstract PDF