Journal of Carcinogenesis & Mutagenesis

Schramm L

Publications

• Research Article
  Gender Specific Differences in RNA Polymerase III Transcription
  Author(s): Diette N, Koo J, Cabarcas-Petroski S and Schramm LDiette N, Koo J, Cabarcas-Petroski S and Schramm L
  
  Background: RNA polymerase (pol) III transcribes a variety of untranslated RNAs responsible for regulating cellular growth and is deregulated in a variety of cancers. In this study, we examined gender differences in RNA pol III transcription in vitro and in vivo. Methods: Expression levels of U6 snRNA, tMet, and known modulators of RNA pol III transcription were assayed in male and female derived adenocarcinoma (AC) lung cancer cell lines and male and female C57BL/6J mice using real time quantitative PCR. Methylation status of the U6 snRNA promoter was determined for lung and liver tissue isolated from male and female C57BL/6J mice by digesting genomic DNA with methylation sensitive restriction enzymes and digestion profiles were analyzed by qPCR using primers spanning the U6 promoter. Results: Here, we demonstrate that RNA pol III transcription is differentially regulat.. View More»
  DOI: 10.4172/2157-2518.1000251

  Abstract PDF