Pharmaceutica Analytica Acta

Safaa T

Publications

• Research Article
  Utility of Synchronous Spectrofluorimetric Method for Rapid Selective Determination of Bimatoprost: Stress Stability Study and Green Analytical Application
  Author(s): Mohammed W, Safaa T, Abou El-Alamin MM, Nahla NS and Elabd MAMohammed W, Safaa T, Abou El-Alamin MM, Nahla NS and Elabd MA
  
  Stability indicating Synchronous spectrofluorimetric (SF) method has been proposed and validated for the determination of bimatoprost in presence of its acid, base, and oxidative degradation products by measuring SF at 272 nm using delta lamba (∆λ)=30 nm in water. Bimatoprost was subjected to stress conditions of 2 M HCL, 2 M NaoH and 30% H2O2 . The identification of degradation products were done by LC-MS. The pathway of degradation was postulated. The effect of different experimental parameters on the fluorescence of the drug was studied and optimized. The range of linearity was over 25-250 ng/mL, the detection limit of 0.005 ng/ mL, and quantitation limit of 0.018 ng/ mL for bimatoprost. The developed method was validated according to ICH guidelines. The accuracy was checked by applying the proposed method for the determination of the drug and it&rsqu.. View More»
  DOI: 10.4172/2153-2435.1000600

  Abstract PDF