Pharmaceutica Analytica Acta

Sadaf Jamal Gilani

Publications

• Research Article
  Quantification and Identification of Bioactive Eugenol in Myristica fragrans Seed Using Validated High Performance Thin Layer Chromatography Technique
  Author(s): Mohamad Taleuzzamana, Asadullah Jahangirb and Sadaf Jamal GilaniMohamad Taleuzzamana, Asadullah Jahangirb and Sadaf Jamal Gilani
  
  A sensitive, selective, precise and stability indicating high-performance thin layer chromatographic method (HPTLC) was developed and validated for analysis of eugenol from methanolic extract of Myristica fragrans seed. The chromatography study was performed on aluminium foil-backed silica gel 60 F-254 HPTLC plates using toluene-ethyl acetate-formic acid (2:5:0.2, v/v/v) as the mobile phase. The densitometric determination was carried out by TLC scanner (CAMAG) at 560 nm in reflectance/absorbance mode. The developed method was validated for different parameters like linearity, precision, recovery, robustness, detection limits, quantification limits and stressed stability study in accordance with ICH guidelines. The developed analytical method were found to be linear in the concentration range of 100-1000 ng band-1 with regression value closer to unity (r2=0.998). The develope.. View More»
  DOI: 10.4172/2153-2435.1000563

  Abstract PDF