S. He
IR Iran
Research Article
Do stocking densities affect the gut microbiota of gibel carp Carassius auratus gibelio cultured in ponds?
Author(s): Z. Zhou, S. He, Y. Liu, P. Shi, B. Yao and E. RingøZ. Zhou, S. He, Y. Liu, P. Shi, B. Yao and E. Ringø
The aim of the present study was to evaluate the intestinal microbial communities of gibel carp ( Carassius auratus gibelio ) cultivated in two beach ponds at different stocking densities. The two ponds were both ~3.33 hm 2 in acreage and ~1.5 m in depth. The stocking densities included one intensive with 2 fish m –3 while the other treated as semi-intensive with 1 fish m –3 . The gut microbiota (both allochthonous and autochthonous) were sampled after 135 days of feeding. Denaturing gradient gel electrophoresis (DGGE) of PCR-amplified 16S rRNA gene segments was used to evaluate the bacterial community. Actinobacteria, Cyanobacteria, Firmicutes, Fusobacteria, Proteobacteria and some unclassified_bacteria taxa were identified in gut samples and feed. Similar bacterial communities ( C s =0.83) were observed with respect to the autochthonous an.. View More»
DOI:
10.4172/2155-9546.S1-003