Ponpisit Utiswannakul
Thailand
Research Article
Enhanced growth of black tiger shrimp Penaeus monodon by dietary supplementation with Bacillus (BP11) as a probiotic
Author(s): Ponpisit Utiswannakul, Siripen Sangchai and Sirirat RengpipatPonpisit Utiswannakul, Siripen Sangchai and Sirirat Rengpipat
Bacillus isolate P11 (BP11), isolated from the gastrointestinal tract of the black tiger shrimp, Penaeus monodon , was evaluated for its potential use as a probiotic feed supplement for P. monodon culture. BP11, a Gram-positive spore forming bacteria, was identified as a member of the genus Bacillus and most likely to be an isolate of Bacillus subtilis , based on biochemical tests, physical morphology, and 16S rRNA gene fragment sequence analysis. BP11 is likely to be safe as a probiotic for P. monodon since no detectable level of antimicrobial substance or Bacillus diarrheal enterotoxin production was found. When the regular feed of P. monodon was supplemented with BP11 at ~10 9 CFU g -1 feed a higher shrimp growth, feed conversion ratio, survival and general health was obtained for both postlarvae (PL) shrimp in concrete tanks and in an earthen .. View More»
DOI:
10.4172/2155-9546.S1-006