Pharmaceutica Analytica Acta

Mitsutoshi Tsukimoto

Publications

• Research Article
  Anti-Angiogenic Effect of P2X7 Receptor Antagonist Oxidized ATP as a Mechanism of Anti-Tumor Growth
  Author(s): Shizuka Seki, Mitsutoshi Tsukimoto, Akina Suzuki, Fumie Hattori, Erina Takai, Yasuhiro Ohshima and Shuji KojimaShizuka Seki, Mitsutoshi Tsukimoto, Akina Suzuki, Fumie Hattori, Erina Takai, Yasuhiro Ohshima and Shuji Kojima
  
  Extracellular ATP accumulated in tumor microenvironment activates P2X7 receptor on cellular membrane of cancer cells. Recently, an importance of P2X7 receptor in cancer growth or malignancy has been suggested. We have reported an inhibitory effect of oxidized ATP (oxATP), which is an irreversible antagonist of P2X7 receptor, on melanoma growth. However, the mechanism has not yet been established. In this study, we investigated the effect of oxATP on angiogenesis in vitro and in vivo to reveal the mechanism of anti-tumor growth by oxATP. We found that oxATP strongly suppressed cell migration and wound healing in mouse endothelium b.End3 cells, indicating the suppressive effect of oxATP on angiogenesis in vitro. We further investigate the effect of oxATP on angiogenesis in vivo. We performed ligation of the femoral artery and vein of BALB/c mouse, and a laser doppler perfusion image ana.. View More»
  DOI: 10.4172/2153-2435.1000190

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