Journal of Microbial & Biochemical Technology

Michiru Kishimoto

Publications

• Rapid Communication
  Combination of Reverse Transcription and Multienzyme Restriction Fragment Length Polymorphism Analysis for Rapid Detection of Escherichia Coli
  Author(s): Akifumi Hosoda, Arata Komaba, Michiru Kishimoto and Hiroto Tamura Akifumi Hosoda, Arata Komaba, Michiru Kishimoto and Hiroto Tamura
  
  Cultivation methods are used to monitor pathogenic microorganisms in foods. However, the current methods require a few days to produce results, and products are often released for sale before the results of microbiological analysis become available. We developed an RNA extraction and microorganism detection system using model food samples inoculated with Escherichia coli K-12 and O157:H7 (GTC 14536) (0 CFU/g and 1×101–104 CFU/g). Before RNA extraction, live or dead cells were inoculated into the food samples, the samples were homogenized, and the extracted RNAs were used to synthesize cDNAs using random 6-mer. PCR was used to analyze the target genes, and the PCR products were digested with two restriction enzymes (HhaI and HaeIII) to analyze restriction fragment length polymorphism (RFLP). PCR confirmed the RNA extraction and cDNA synthesis of up to 1×101 CFU/g samp.. View More»
  DOI: 10.4172/1948-5948.1000113

  Abstract PDF