Journal of Aquaculture Research & Development

Matthias Monfort

Publications

• Research Article
  An ex vivo approach to studying the interactions of Pediococcus acidilactici and Vibrio (Listonella) anguillarum in the anterior intestine of rainbow trout Oncorhynchus mykiss
  Author(s): Glenn M. Harper, Matthias Monfort, Imad P. Saoud, Matthew J. Emery, Sanaa Mustafa, Mark D. Rawling, Ben Eynon, Simon J. Davies and Daniel L. Merrifield Glenn M. Harper, Matthias Monfort, Imad P. Saoud, Matthew J. Emery, Sanaa Mustafa, Mark D. Rawling, Ben Eynon, Simon J. Davies and Daniel L. Merrifield
  
  The aim of the present study was to observe the antagonistic relationship of the probiotic Pediococcus acidilactici and the pathogen Vibrio (Listonella) anguillarum in the intestine of rainbow trout (Oncorhynchus mykiss) by using the ex vivo intestinal sac method. Rainbow trout (240-250g) were fed either a control diet (commercial diet: 43% protein, 20% lipid) or a probiotic diet (control diet supplemented with P. acidilactici [Bactocell] at log 7 CFU g-1) for two weeks. The anterior intestine was then isolated for an ex vivo challenge study and intestinal sacs formed by shutting one end of the intestinal portion. The sacs were filled with PBS solutions containing either no bacteria (as a control), the probiotic (P. acidilactici), the pathogen V. anguillarum, or probiotic+pathogen and incubated for one hour. At the end of the exposure bacterial levels.. View More»
  DOI: 10.4172/2155-9546.S1-004

  Abstract PDF