Journal of Blood Disorders & Transfusion

Maria Liljeholm

Publications

• Research Article
  Erythrocyte Flow Cytometric Analysis in Congenital Dyserythropoietic Anemia Type III-Evaluation of Eosin-5 ́ -Maleimide, CD55, and CD59
  Author(s): Maria Liljeholm, Elisabeth Gronlund, Irina Golovleva, Herbert Sandström and Anders WahlinMaria Liljeholm, Elisabeth Gronlund, Irina Golovleva, Herbert Sandström and Anders Wahlin
  
  Introduction: Flow cytometry with eosin-5´-maleimide (EMA), anti-CD55 and anti-CD59 is commonly used when investigating non-autoimmune hemolytic anemias. Reduced fluorescence of EMA, typically detected in hereditary spherocytosis is also seen in congenital dyserythropoietic anemia type II (CDA II). Reduction of CD55 and CD59 characterizes paroxysmal nocturnal hemoglobinuria (PNH). We studied the flow cytometric profile of EMA, CD55 and CD59 on erythrocytes in congenital dyserythropoietic anemia type III (CDA III). Methods: Erythrocytes from 16 CDA III positive individuals, 14 CDA III negative relatives and three normal controls per assay were studied with flow cytometry after EMA staining. Flow cytometry after anti-CD55 and anti- CD59 was performed on erythrocytes from 12 CDA III positive and 7 CDA III negative relatives with one normal control per assay. Results: CDA III - eryt.. View More»
  DOI: 10.4172/2155-9864.1000172

  Abstract PDF