Journal of Carcinogenesis & Mutagenesis

Leon LB

Publications

• Research Article
  Radio sensitization of Colon Cancer Cells Mediated by Gemcitabine through Cell Cycle Synchronization
  Author(s): Zanandrea M, Baes TW, Leon LB, Amado GV, Reis VS, Filho AB, Rocha AB and Grivicich IZanandrea M, Baes TW, Leon LB, Amado GV, Reis VS, Filho AB, Rocha AB and Grivicich I
  
  We evaluated gemcitabine together with ionizing radiation for improved cell growth inhibition with respect to that by radiation alone in the human colon carcinoma cell lines SW620, HT-29 and SNU-C4. To this end, cells were exposed for 24 h to gemcitabine and then assessed for growth response with the sulphorhodamine B assay. The cell lines, as well as exposed to ionizing radiation and a combination of gemcitabine and ionizing radiation for 24 h, 48 h and 72 h and the radiosensitivity was assessed using a clonogenic assay. Multiple drug effect analysis was used to evaluate the synergistic effect, which was then related to the cell cycle phase distribution. The SNU-C4 cell line showed a greater sensitivity to gemcitabine in comparison to the other two cell lines, while the SW620 cells was more sensitive to damage induced by radiation. Furthermore, gemcitabine increased by 50% the effect.. View More»
  DOI: 10.4172/2157-2518.1000239

  Abstract PDF