Journal of Carcinogenesis & Mutagenesis

Jiangdong Wang

Publications

• Research Article
  A Superficial Colon Tumor Model Involving Subcutaneous Colon Translocation and Orthotopic Transplantation of Green Fluorescent Protein-Expressing Human Colon Tumor
  Author(s): Heiying Jin, Zhijian Yang, Jiangdong Wang, Suying Zhang, Yu Sun and Yijiang DingHeiying Jin, Zhijian Yang, Jiangdong Wang, Suying Zhang, Yu Sun and Yijiang Ding
  
  Background: The orthotopic transplantation model of human tumor has been demonstrated to be more patientlike animal tumor model. However, observations of tumor progression and metastasis are limited by the deep location of the colon or limited deep penetration ability of fluorescence through tissue. The purpose of this study is to establish a superficial orthotopic model to allow easier real-time visualization and more sensitive monitoring of fluorescent orthotopic colon tumor. Methods: Human colon cancer HT-29 cells were transduced with a pLPCX expression retroviral vector containing GFP and neomycin resistance genes. For superficial orthotopic transplantation model, the cecum was identified and pulled out of the peritoneal cavity, the space between the cecum and peritoneum was sutured, the cecum was pulled to subcutaneous tissue, and an incision was.. View More»
  DOI: 10.4172/2157-2518.1000104

  Abstract PDF