Biochemistry & Analytical Biochemistry

Jarosław Błaszczyk

Publications

• Research Article
  Handling of Selenomethionines in Macromolecular Refinement
  Author(s): Jarosław BłaszczykJarosław Błaszczyk
  
  In the Protein Data Bank, we can find X-ray structures which were determined from selenium-derivatized proteins, but the deposited coordinates, to our surprise, contain native methionines. The problem is likely due to not complete substitution of native methionines with selenomethionines during chemical reaction. When the crystal grows from such sample, and later is subjected for X-ray analysis, it may show partial presence of native sulfur. This paper is a voice in discussion how to handle the refinement of selenomethionines. The purpose of this work is to explain why selenomethionines can’t be refined purely as native methionines, particularly in macromolecular structures determined at high resolution. It is important because selenium and sulfur are different chemical elements. To explain the importance of refinement of correct atom type, author provides evidence, collected ma.. View More»
  DOI: 10.4172/2161-1009.1000155

  Abstract PDF