Iván Murillo
Colombia
Research Article
Bacillus cereus and Bacillus subtilis used as probiotics in rotifer (Brachionus plicatilis) cultures
Author(s): Iván Murillo and Luisa Villamil
Iván Murillo and Luisa Villamil
Since live food is a key factor in fish and invertebrate larvae rearing, the objective of this study was to evaluate the probiotic potential of two Gram positive Bacillus species: Bacillus cereus (CCBM-2) and Bacillus subtilis (CCBM- 64), originally isolated from marine sediments in the Colombian Caribbean, in rotifer Brachionus plicatilis cultures. The enzymatic activity and antibacterial activity against pathogens of B. cereus and B. subtilis were measured in vitro. Direct supplementation of the rotifer culture water with both bacteria was also carried out and the effect was measured in vivo by estimating the density of rotifers and bacterial counts in the culture. The results indicated that extracellular products of both bacteria strongly inhibited the growth of AeromonA. hydrophila and Vibrio alginolyticus isolated from diseased fish. Enzyme API Z.. View More»
DOI:
10.4172/2155-9546.S1-007