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Ichihara H
Ichihara H
Japan
Publications
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Research Article
Negatively Charged Cell Membranes-Targeted Highly Selective Chemotherapy with Cationic Hybrid Liposomes against Colorectal Cancer In Vitro and In Vivo
Author(s): Ichihara H, Motomura M and Matsumoto YIchihara H, Motomura M and Matsumoto Y
Cationic hybrid liposomes (CHL) composed of 87 mol% L-α-dimyristoylphosphatidylcholine (DMPC), 5 mol% polyoxyethylene (21) dodecyl ether (C12(EO)21) and 8 mol% O,O’-ditetradecanoyl-N-(α–trimethylammonioacetyl) diethanolamine chloride (2C14ECl) were prepared by the method of sonication. A clear solution of CHL having a hydrodynamic diameter of 100 nm could be maintained over 4 weeks. The IC50 value of CHL on the growth of human colorectal cancer (CRC; HCT116) cells was remarkably smaller than that of the DMPC liposomes. Immediate fusion of CHL including NBDPC into HCT116 cell membranes was confirmed using a confocal laser and total internal reflection fluorescence (TIRF) microscope without affecting normal colon (CCD-33Co) cells. Activation of caspases for apoptosis of HCT116 cells induced by CHL was verified on the basis of fluorescence microscopy. Carcinoma HC.. View More»
DOI: 10.4172/2157-2518.1000267