Biochemistry & Analytical Biochemistry

Evgeny A Ermakov

Publications

• Research Article
  Identification of Major Proteins of a Very Stable High Molecular Mass Multi-Protein Complex of Human Placental Tissue Possessing Nine Different Catalytic Activities
  Author(s): Evgeniya E Burkova, Pavel S Dmitrenok, Dmitrii V Bulgakov, Evgeny A Ermakov, Valentina N Buneva, Svetlana E Soboleva1 and Georgy A Nevinsky*Evgeniya E Burkova, Pavel S Dmitrenok, Dmitrii V Bulgakov, Evgeny A Ermakov, Valentina N Buneva, Svetlana E Soboleva1 and Georgy A Nevinsky*
  
  Human placenta is an organ protecting, feeding, and regulating the grooving of the embryo. Therefore, identification and characterization of placental components including proteins and their multi-protein complexes is an important step to understanding the placenta function. Here we analyzed for the first time human placentas extremely stable multi-protein complex (SPC, ~1000 kDa) by MALDI MS and MS/MS spectrometry using proteins tryptic hydrolyzates after proteins separation by SDS-PAGE and 2-D electrophoresis. The formation of such a very stable complex due to the random association of several various proteins is very unlikely. It was shown that SPCs contain twelve proteins: hemoglobin, alkaline phosphatase, cytoplasmic actin, human serum albumin, chorionic somatomammotropin hormone, heat shock protein beta-1, peroxiredoxin-1, 78 kDa glucose-regulated protein, protein disulfide.. View More»
  DOI: 10.4172/2161-1009.1000351

  Abstract PDF