Pharmaceutica Analytica Acta

Das S

Publications

• Research Article
  Simultaneous Determination and Mutual Interaction Study of Ciprofloxacin and Chloramphenicol in Concomitant Administration by a New UPLC Method
  Author(s): Uddin MN, Das S, Md Mijan NH, Md Al-Amin and Bhuiyan HRUddin MN, Das S, Md Mijan NH, Md Al-Amin and Bhuiyan HR
  
  A new simple, fast, accurate, precise and reproducible UPLC method was developed for the simultaneous estimation of ciprofloxacin and chloramphenicol. Reversed-phase Shim-pack XR-ODS (100 × 3.0 mm, 1.7 μm) column was used to resolve the drugs utilizing a mixture of CH3OH and 5 mM NaH2PO4 as mobile phase at gradient program equilibrated initially by 56:44 (v/v). The mobile phase was pumped at a flow rate of 0.20 mL min-1 with detection at 280 nm. 10 μL volume of sample was injected by the auto sampler. Separation was completed within 3.82 ± 0.03 minutes. For both drugs linear was obtained over a studied concentration range of 10 μg mL-1 with correlation coefficient 0.999. Relative Standard Deviation (RSD) for intra and inter day precision was <1.5% indicating the method’s good reproducibility. The mean recovery of the drug determination was 99.39%. LOD wa.. View More»
  DOI: 10.4172/2153-2435.1000535

  Abstract PDF