Boost WCS
Hong Kong
Research Article
To Study the Effects of Shenqifuzheng Injection (SFI) on Micro-RNAs in Human Dendritic Cells
Author(s): Yuwh H, Sze WH, Yip DMY, Cho SP, Boost WCS, Zhang MV, Fan Z, Ng K, Ng MCH, Yeung JWC, Hung A, H WH, Chong GSL and Lee RLPYuwh H, Sze WH, Yip DMY, Cho SP, Boost WCS, Zhang MV, Fan Z, Ng K, Ng MCH, Yeung JWC, Hung A, H WH, Chong GSL and Lee RLP
Method: This study used a 2-step approach. By first we were using a robust DC cell line to identify those miRNA that might be involved in DC activation; then followed by confirmation using monocyte-derived DC which was closer to real-life physiological contexts. THP-1 cell, a human monocytic leukemia cell line, has previously been shown to be useful for the in vitro study of DC. In this study, we identified a number of potential miRNA candidates in the DCs activated by SFI using MicroRNA Array of 381 known human miRNAs (Sanger miR Base, version 14). miR-22, miR-107 and miR-200a were then selected from the over 30 potential miRNA candidates with relative expression fold change >2 indentified in accordance to the computational evidences retrieved in the annotation database pipeline miR Base. These three miRNAs were further examined for any change in expression in a confirmatory study.. View More»
DOI:
10.4172/2153-0602.1000149