Journal of Stem Cell Research & Therapy

Anongnad Ngamjariyawat

Publications

• Research Article
  Co-culture of Insulin Producing Human EndoC- βH1 Cells with Boundary Cap Neural Crest Stem Cells Protects Partially against Cytokine-induced Cell Death
  Author(s): Anongnad Ngamjariyawat, Svitlana Vasylovska, Kyril Turpaev, Phillippe Ravassard, Nils Welsh, Elena Kozlova and Rikard G FredAnongnad Ngamjariyawat, Svitlana Vasylovska, Kyril Turpaev, Phillippe Ravassard, Nils Welsh, Elena Kozlova and Rikard G Fred
  
  We have recently observed that co-culture of mouse and rat beta-cells with mouse boundary cap neural crest stem cells (bNCSCs) protected against inflammatory cytokine-induced beta-cell death, possibly via direct cadherinmediated cell-to-cell junctions. However, it has not been addressed whether also human beta-cells can be protected via this strategy. If possible it would be an important approach for development of new protocols for improved outcome of islet transplantation to Type-1 diabetes patients. The aim of this investigation was therefore to study the effect of bNCSC co-culture with insulin producing human EndoC-βH1 cells on cytokine-induced cell death. For this purpose GFP-positive bNCSCs were cultured together with GFP-negative human EndoC-βH1 cells in the presence of the cytokines IL-1γ (50 U/ml) and IFN-γ (1000 U/ml). Cells were then stained with propi.. View More»
  DOI: 10.4172/2157-7633.1000343

  Abstract PDF