Biology and Medicine

Amelework Eshetu

Publications

• Research Article
  Evaluation of Diuretic Activity of Methanol Crude Extract of Thymus serrulatus Leaves and its Solvent Fraction in Mice
  Author(s): Amelework Eshetu, Eyasu Makonnen, Asfaw Debella, Netsanet Fekadu and Bekesho GeletaAmelework Eshetu, Eyasu Makonnen, Asfaw Debella, Netsanet Fekadu and Bekesho Geleta
  
  Background: Thymus serrulatus is an indigenous plant to Ethiopia and used widely in the treatment of many illnesses including renal diseases and hypertension. The present study was undertaken to investigate the diuretic activity of the 80% methanol crude extract and solvent fractions of T. serrulatus leaves in saline-loaded mice. Methods: Swiss albino mice of either sex were divided into six groups (five animals in each group). The control group received normal saline (25 ml/kg) in 2% Tween 80, the reference group received hydrochlorothiazide (10 mg/ kg). Group-III to Group-XIV received the test substances at dose levels of 125, 250, 500, and 1000 mg/kg orally. At the end of the 5th hour, urine was collected and the total volume of urine excreted by each animal was recorded. Concentration of urinary Na+, K+, and Na+/K+ ratio were also determined. The acute toxicity test was conducted .. View More»
  DOI: 10.4172/0974-8369.1000347

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