Pharmaceutica Analytica Acta

Akbar F

Publications

• Research Article
  Development and Validation of Analytical Method for Quantification of Losartan Potassium in Solid Dosage Form
  Author(s): Latif A, Akbar F, Khan AJ, Shafi H and Mazhar MLatif A, Akbar F, Khan AJ, Shafi H and Mazhar M
  
  The aim of this study was to develop and validate a simple, robust, reliable and an accurate isocratic reverse phase high-performance liquid chromatography (RP-HPLC) method for quantification of Losartan potassium in solid dosage form using DAD Detector. Elution was carried out with mobile phase comprising of 0.01 M monobasic potassium dihydrogen phosphate buffer (adjusted at pH 3.0 ± 0.05 with ortho-phosphoric acid) and methanol (40: 60), through octadecyl silyl (C18) column (15 cm x 4.6 mm x 5 μl), at flow rate of 1 ml/min. The detection was carried out at 230 nm. The developed method was validated according to International Conference on Harmonization (ICH) guidelines (ICH 2005). The assay was linear in concentration range of 1-3 µgmL-1 with Correlation coefficient of 0.999. The limit of detection was 0.036 µgmL-1 and limit of quantification was 0.1.. View More»
  DOI: 10.4172/2153-2435.1000592

  Abstract PDF