It is common practice for many athletes and active adults to use protein supplements to enhance gains in lean muscle mass, and whey protein, in particular, is ideal due to its high levels of essential amino acids. Whey protein isolates, one of the most common forms of protein, are considered one of the best sources of protein available. To date, no studies have examined the relative performance of whey protein that has undergone the patent-pending Ingredient Optimized process in order to enhance its effects on muscle protein synthesis and lean muscle anabolism. As such, the primary aim of this study was to examine the relative benefit of this ioProtein whey protein isolate, in combination with resistance exercise, on several measures of body composition among a sample of athletes in comparison with those associated with an untreated whey protein isolate. Twenty healthy male and female adults, between the ages of 18 and 37 years, were recruited. Results indicated that individuals who supplemented with the Ingredient Optimized whey protein saw a significant improvement in fat-free mass (p<0.5) compared to those taking non-optimized, non-treated whey protein. Moreover, individuals supplementing with Ingredient Optimized whey protein also experienced significantly enhanced performance on bench press (p<0.5), squat (p<0.5), recovery time (p<0.5), and stomach discomfort (p<0.5), which was not reported from individuals taking the non-optimized control whey protein.