Clinical Microbiology: Open Access

Abstract

Genotyping of soil and clinical isolates of Burkholderia species in Myanmar

Nay Myo Aung*, Khine Khine Su, Narisara Chantratita, Chanwit Tribuddharat

Burkholderia species are known as medically necessary pathogens not only in a clinical setting but environmental sectors. Cost-effective ways of molecular genotyping among Burkholderia species in Myanmar were found to be limited and low awareness. In this study, 21 clinical and 29 soil isolates were collected and performed to discriminate Burkholderia species. Two genotyping methods were performed, recA, 16S rRNA amplification and sequencing. Phylogenetic trees were constructed using sequences of recA and 16S rRNA genes. Twenty-nine soil isolates resulted as 5 Burkholderia pseudomallei, 5 Burkholderia cepacia, 13 Burkholderia thailandensis, 4 Burkholderia mutivorans and 2 Burkholderia cenocepacia. Sixteen clinical and 5 soil isolates of Burkholderia pseudomallei were taken and assessed 16S rRNA type. RecA sequencing was grouped into 5 clades, while 16S rRNA sequencing showed two. The more type ability and discriminatory power of recA were found than 16S rRNA sequencing among Burkholderia species. The recA sequencing showed a greater discriminatory power and type ability, suggesting that it may be a more reliable and helpful tool for identifying and classifying Burkholderia species. Therefore, recA sequencing can be considered a better alternative to 16S rRNA sequencing for the molecular characterization of Burkholderia species in Myanmar.

Published Date: 2025-04-11; Received Date: 2024-02-09