Global Journal of Biology, Agriculture & Health Sciences

Abstract

ELECTROPORATION OF snoRNA IN Giardia lamblia

Sumallya Karmakar, Avik Kumar Mukherjee, & Sandipan Ganguly

Electroporation is a significant increase in the permeability of the cell membrane caused by an externally applied electrical field. It is used in molecular biology for the introduction of various micromolecular substances into a cell. Compared to other biochemical techniques, electroporation is simple, easily applicable and very efficient. This study reports the electroporation of invitro transcribed giardial snoRNA into Giardia lamblia by varying the number of pulsed under a constant electric field and its visualisation using a confocal microscope.

Published Date: 2013-09-19;