Journal of Coastal Zone Management

Abstract

Antibacterial Activity of A Secondary Metabolite-Producing Coral Bacterium Pseudoalteromonas species

Ocky Karna Radjasa, Torben Marten, Thorsten Brinkoff,Hans-Peter Grossart, Agus Sabdono and Meinhard Simon

A bacterium, collected at the surface of coral Acropora sp., TAB4.2 was successfully screened for
secondary metabolites production based on PCR amplification of the non-ribosomal peptide synthetase
gene. It was identified as closely related to Pseudoalteromonas luteoviolacea based on its 16S rDNA.
TAB4.2 was found to inhibit the growth of all 5 coral-associated and all 5 pathogenic bacteria tested.
To characterize the inhibiting metabolite, a 279 bp long DNA fragment was obtained and the deduced
amino acid sequence showed conserved signature regions for peptide synthetases and revealed a high
similarity to NosD (40 % identity), a multifunctional peptide synthetase from Nostoc sp. GSV224, and
NdaB (44 % identity), a peptide synthetase module of Nodularia spumigena