Journal of Vaccines & Vaccination

Vaccination with recombinant 4Ã?M2e.HSP70c fusion protein as a universal vaccine candidate enhances both humoral and cell-mediated immune responses and decreases viral shedding against experimental challenge of H9N2 in

11^th Global Summit and Expo on Vaccines, Vaccination and Therapeutics

September 12-14, 2016 Phoenix, USA

Mashallah Mohammadi

Razi Vaccine and Serum Research Institute, Iran

Accepted Abstracts: J Vaccines Vaccin

Abstract:

As cellular immunity is essential for virus clearance, it is commonly accepted that no adequate cellular immunity is achieved by all available inactivated HA-based influenza vaccines. Thus, an improved influenza vaccine to induce both humoral and cell-mediated immune responses is urgently required to control LPAI H9N2 outbreaks in poultry farms. M2e-based vaccines have been suggested and developed as a new generation of universal vaccine candidate against influenza A infection. Our previous study have shown that a prime-boost administration of recombinant 4�?M2e.HSP70c (r4M2e/H70c) fusion protein compared to conventional HA-based influenza vaccines provided full protection against lethal dose of influenza A viruses in mice. In the present study, the immunogenicity and protective efficacy of (r4M2e/H70c) was examined in chickens. The data reported herein show that protection against H9N2 viral challenge was significantly increased in chickens by injection of r4M2e/H70c compared with injection of conventional HA-based influenza vaccine adjuvanted with MF59 or recombinant 4�?M2e (r4M2e) without HSP70c. Oropharyngeal and cloacal shedding of the virus was detected in all of the r4M2e/H70c vaccinated birds at 2 days after challenge, but the titer was low and decreased rapidly to reach undetectable levels at 7 days after challenge. This protective immunity might be attributed to enhanced cell-mediated immunity, which is interpreted as increased lymphocytes proliferation, increased levels of Th1-type (IFN-) and Th2- type (IL-4) cytokines production and increased CD4+ to CD8+ ratios, resulting from the injection of four tandem repeats of the ectodomain of the conserved influenza matrix protein M2 (4�?M2e) genetically fused to C-terminus of Mycobacterium tuberculosis HSP70 (mHSP70c).

Biography :

Email: mashallah8@yahoo.com