Journal of Bacteriology & Parasitology

Vaccination with a multi-epitope protein vaccine encoding T and B-cell epitopes of Trichinella spiralis paramyosin elicits partial protection in mice

International Conference on Medical Parasitology and Zoology

October 17-19, 2016 Houston, USA

Yuan Gu

Capital Medical University, China

Scientific Tracks Abstracts: J Bacteriol Parasitol

Abstract:

Trichinellosis is a world widespread zoonosis caused by infection of Trichinella spiralis (T. spiralis) through ingesting contaminated pork or other meat. Interrupting parasite transmission via vaccination of livestock with potent vaccine is a practical approach to prevent trichinellosis in China. Paramyosin of T. spiralis (Ts-Pmy) has been proved to be a good vaccine candidate against trichinellosis according to our previous work. As humoral immunity plays an important role in the elimination of the parasite, a synthetic gene containing a protective B-cell epitope and four Th2 epitopes of Ts-Pmy identified before was cloned into the eukaryotic expression vector pET-28a and the recombinant multi-epitope protein (rMEP) was purified. Mice immunized with rMEP formulated with ISA50v2 adjuvant exhibited a significant reduction in muscle larvae burden (55.4%) after a challenge with T. spiralis compared to the adjuvant control group (p<0.01). The rMEP-induced protection was associated with a high level of specific anti-rMEP IgG antibodies and a Th1/Th2 mixed immune response. This research laid a foundation for designing effective epitope-based multivalent subunit vaccine against trichinellosis.

Biography :

Yuan Gu has completed her PhD in 2007 from Capital Medical University, China. She is an Associate Professor of Capital Medical University. She has published about 15 papers in reputed journals and two patents have been granted.

Email: guyuan@ccmu.edu.cn