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Understanding mechanism of action of neutralizing antibodies helps guide virus-like particle construction
6th Euro Global Summit and Expo on Vaccines & Vaccination
August 17-19, 2015 Birmingham, UK
David Lawrence
Posters-Accepted Abstracts: J Vaccines Vaccin
Abstract:
The life cycle of human enteroviruses generates several different particle types including the procapsid, the infectious particle,
and the expanded particle. All these particles are immunogenic but understanding the mechanism, by which antibody
neutralization takes place, is crucial to predicting whether the particles will be protective. We present two different neutralizing
antibodies against human enterovirus 71 (EV-A71) that recognizes epitopes comprising more than one capsid protein in a
quarternary structure. The footprint of each antibody on the virion is demonstrated using cryo EM. The antibodies were used
to characterize and purify virus-like particles that were effectively procapsids, generated using recombinant baculoviruses. In
both mouse and non-human primate studies, the purified particles were shown to elicit strong neutralizing antibodies against
EV-A71. An assay to identify and quantify these particles is described.