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To study the molecular characterization of elite cotton germplasm using DNA marker
3rd International Conference on Agriculture & Horticulture
October 27-29, 2014 Hyderabad International Convention Centre, India
Sham Chavan, H V Kalpande, S K Chavan, J D Deshmukh, V S Patil, T Anjaneyulu and V R Rachiyata
Posters: Agrotechnol
Abstract:
Inter simple sequence repeat DNA (ISSR) marker was used for molecular evaluation of twelve elite cotton genotypes to Gossypium hirsutam. The dendrogram generated by ISSR marker showed two clusters of the genotypes of Gossypium hirsutam brought from Dharwad, Nanded and Parbhani. Dendrogram obtained by ISSR marker showed similarity coefficient value in the range of 0.32 to 0.80. Two cotton genotypes NDH- 58 and NDH - 88 showed the highest similarity, whereas, genotype PH - 1009 showed least similarity coefficient of 0.428. ISSR primer IS - 12, IS - 13 generated maximum number of amplicons i.e. eight and IS -2, IS - 14 primers generated least number of amplicons i.e. four.