Journal of Microbial & Biochemical Technology

Thermo-anaerobic solubilization mechanism of a phosphate rock by Bacillus subtilis during a grassland biodegradation process

Joint Event on 4^th World Congress and Expo on Applied Microbiology & 2^nd International Conference on Food Microbiology

November 29-December 01, 2017 Madrid, Spain

Hanane Hamdali, Moussa Seyni Hassimi, Yedir Ouhdouch, Eric Pinelli, George Merlina and Mohamed Hafidi

Laboratory of Genetic and Plant Biotechnology, Faculty of Sciences and Technology, University of Sultan Moulay Slimane, B.P : 523 Beni Mellal, Morocco
Faculté de Sciences Semlalia, Université Cadi Ayyad (UCAM), Laboratoire d�??Ecologie et Environnement(L2E) (Unité Associée au CNRST, URAC32, Unité associée au CNRS), Marrakech Morocco
Faculte de Sciences Semlalia, Universite Cadi Ayyad (UCAM), Laboratoire de Biologie et de Biotechnologie des Microorganismes, Marrakech Morocco
Ecolab-UMR 5245 CNRS-UPS-INPT, Toulouse France
Departement Sciences de l'environnement et Adaptation aux Changements Climatiques, Universite de Tillaberi, Niger

Posters & Accepted Abstracts: J Microb Biochem Technol

Abstract:

Bacillus subtilis isolated from the cellulosic fermentation process was previously selected for its phosphate rock (PR) solubilization ability under anaerobic thermophilic conditions. The result shows a significant decrease of pH from 5.8 to 4.6, and solubilizing 38% of the phosphorus from the PR in the reactors after 30 days of incubation at 50°C. This solubilization is the consequence of the production of organic acids during the anaerobic fermentation of grassland substrate. The mechanisms involved in these weathering processes confirmed the production of organic acids which were identified and quantified. We also tested the dissolution of PR by leaching with organic acids trade predominantly present in the acidogenic stage during the anaerobic degradation of lignocellulosic substrate by Bacillus subtilis. The study focused on acetic, lactic and citric acids with varied concentration by measuring pH and ortho-P released monitoring during PR leaching process. The result showed that citric acid exhibited the highest PR dissolution rate (98%, T23h) compared to the other tested acids. By this way, we expect to lead to the development of novel, non-polluting farming practices by entering in the formulation of novel multi-functional biofertilizer by inoculating this thermo tolerant phosphate solubilizing bacterium into agricultural wastes as a practical and environmental strategy.