Journal of Bacteriology & Parasitology

The use of SD BIOLINE TB Ag MPT 64 detection assay for rapid characterization of mycobacteria in Nigeria

3^rd International Congress on Bacteriology and Infectious Diseases

August 04-06, 2015 Valencia, Spain

Ibrahim S1, U B Abubakar1, Danbirni S1, Usman A1, Kudi A C1, Lawson L2, Habib A3 and Abdulkadir I A1

Posters-Accepted Abstracts: J Bacteriol Parasitol

Abstract:

Laboratories performing culture and characterization of Mycobacteria are very few in low resource settings like Nigeria; this
is largely due to stringent requirement of biosafety measures, longer turnaround time and laborious nature of tests. Hence,
a rapid, simple and accurate test for characterization is needed. The “SD BIOLINE TB Ag MPT 64 RAPID” is a simple and
rapid immune chromatographic test used in differentiating Mycobacteria into Mycobacteria tuberculosis complex (MTBC)
and non-tuberculous Mycobacteria (NTM). 100 sputa were obtained from patients suspected to be infected with tuberculosis
and presented themselves to the selected the hospitals for check-up and treatment were involved in this study. The samples
were cultured in a class III Biosafety cabinet and level III biosafety practices were followed. 40 isolates were obtained from
the cultured sputa and they were identified as Acid-fast Bacilli (AFB) using Zeihl-Nelsen acid-fast stain. All the isolates (AFB
positive) were then subjected to the SD BIOLINE Analysis. 31 (77.5%) were characterized as MTBC while 9 (22.5%) were
NTM. The total turnaround time for the rapid assay was just 30 minutes as compared to few days for phenotypic and genotypic
methods. It was concluded that “SD BIOLINE TB Ag MPT 64 Rapid” is simple, rapid and reliable test to differentiate MTBC
from NTM.