Journal of Bacteriology & Parasitology

The use of different laboratory methods in diagnosis of Helicobacter pylori infection: A comparative study

International Congress on Bacteriology & Infectious Diseases

November 20-22, 2013 DoubleTree by Hilton Baltimore-BWI Airport, MD, USA

Nermin H. Ibrahim, Azza Abdulazim Gomaa, Mohamed Ahmed Abu-Sief, Tamer M. Hifnawy and Mervat Abd El-Baseer Tohamy

Scientific Tracks Abstracts: J Bacteriol Parasitol

Abstract:

Background: Helicobacter pylori is well recognized as a major cause of gastrointestinal illnesses and gastric cancers. Therefore, the current study aimed to assess different methods for detection of H. pylori in the oral cavity (saliva and dental plaque) and in gastric biopsy among patients with gastric affection, as well as, detection of H. pylori antigen in stool, moreover, to evaluate the antibiotic susceptibility testing of the isolated strains. Methods: Specimens were obtained from Endoscopy Unit, Internal Medicine Department, Faculty of Medicine, Beni Suef University Hospital, Egypt. Thirty patients were subjected to detailed history and different sampling; gastric biopsy, oral and stool samples. The oral and gastric samples were processed and cultured. Thereafter, microscopic examination and rapid urease tests (RUTs) were conducted. H. pylori antigen detection was carried out in the stool samples, as well as, susceptibility testing to several antibiotics for all isolates identified. Results: The selected patients had a mean age of 36.23+6.317 years. They included 17 males (56.7%) and 13 females (43.3%). 90% of the cases were found positive by culture of the gastric biopsies, while, 96.7% were positive in oral cultures. 92.5% of the gastric samples showed positive results by microscopic examination, however, RUTs were positive in 63.3% of the gastric samples and in 73.3% of the oral samples, meanwhile, 66.7% of patients were found positive by testing their stool for H. pylori antigens. The prevalence of resistance among gastric and oral isolates to amoxycillin, amoxycillin/clavulinic acid, ampicillin/sulbactam, clarithromycin, tetracycillin and metronidazole were; (3.7 and 17.2%), (11.1 and 24.1%), (11.1 and 20.7%), (11.1 and 24.1%), (25.9 and 37.9%) and (96.3 and 100%) respectively. Conclusion: There is an evidenced association between gastric affection and oral H. pylori recognition that, even exceeds stool detection of H. pylori antigen. Moreover, continuous evaluation of antibiotic susceptibility should be carried out and clinicians should be aware about it to select the appropriate empiric regimen for H. pylori eradication.