The Emergence of OXA-48-like and NDM-1-producing Klebsiella pneumoniae in Southern of Turkey
3rd International Congress on Bacteriology and Infectious Diseases
August 04-06, 2015 Valencia, Spain

Farzad Heydari, Tülin Güven Gökmen, Mahdi Marzi and Fatih Köksal

Posters-Accepted Abstracts: J Bacteriol Parasitol


The Emergence of OXA-48-like and NDM-1-producing Klebsiella pneumoniae in Southern of Turkey: New Delhi metallo-
β-lactamase-1 (NDM-1), an acquired class B carbapenemase, is a significant clinical threat due to its extended hydrolysis of
β-lactams including carbapenems. The aim of this study was the detection of MBLs in Enterobacteriaceae isolated from patients
in south of Turkey. 100 Klebsiella pneumoniae were recovered from hospitalized patients in the different units of Çukurova
University Hospital in Adana, Turkey. PCR method was used to detect the extended spectrum beta-lactamase genes (blaTEM,
blaSHV) and carbapenemase genes (blaKPC, blaVIM, blaIMP, blaNDM, and blaOXA-48-like). Clonal relationship was assessed by pulsedfield
gel electrophoresis (PFGE). The studied isolates of K. pneumonia carrying the blaNDM-1 gene were resistant to imipenem,
meropenem, and a high rate of co-resistance to the other antibiotics. These blaNDM-1 carrying isolates remained susceptible to
colistin. The nine K. pneumoniae harbored to blaNDM-1. In a total of nine blaNDM-1 positive isolates, five isolates were positive to
blaOXA-48-like, four isolates were positive to blaVIM, six isolates were positive to blaTEM and eight isolates were positive to blaSHV.
None of the isolates harbored blaKPC or blaIMP. The findings confirm that the rapid spread of NDM-1-producing Gram negatives
is becoming a major challenge for the treatment and control of healthcare-associated infections in our geographic area. The
result of the study reports the emergence of the K. pneumoniae carrying NDM-1 gene which exhibited resistance to imipenem
and meropenem for the first time in southern of Turkey.