Journal of Bacteriology & Parasitology

The effect of fermented milk with Bifidobacteriumon intestinal disorders in the case of contamination with Enteropathogenic Escherichia coli (EPEC)

3^rd International Congress on Bacteriology and Infectious Diseases

August 04-06, 2015 Valencia, Spain

F Mahmoudi and M Hedadji

Posters-Accepted Abstracts: J Bacteriol Parasitol

Abstract:

This study illustrates the importance of antimicrobial Bifidobactérium.sp against the enteropathogens. Six strains of
bifidobacteria belonging to the species B. bifidum, B. longum, and B.breve) were isolated on MRS medium supplemented
with 0.05% cysteine chlorhidrique and 2 mg/l nalidixic acid from stools of infants (their ages less than 5 months), yogurt
(Activia ) and reveald solution of déshydratation (Celia). The sensitivity of strains to antibiotics was also evaluated with
30 antibiotics, this strain are resistant to nalidixic acid, neomycin, vancomycin. The results of the antimicrobial activity
against Gram-positive bacteria and gram-negative revealed that most strains of Bifidobacterium isolated produce and
excrete into the culture medium inhibitory substances capable of inhibiting growth (Staphylococcus aureus, Escherichia
coli, Pseudomonasaeruginosa, salemonella.sp, Enterobacter claoqui). The activity was destroyed by the action of proteolytic
enzymes, indicating that the biologically active portion is proteinaceous. These properties suggest that the inhibitory substance
is considered like substance “bacteriocin”. The study of the kinetics of growth in mixed culture allowed us to choose a strain
of B.Bifidum as a model strain, for antagonism in vivo developed in the rat(wistar), watch it there’s a strong possibility
that the strain has resisted the gastro- intestinal passage. Similarly, in this work, we also records that the administration of
Bifidobacterium strain cause a significant reduction in the rate of E. coli enteropathogenic in the feces of rats and the results
of the macroscopic study of histological sections showed the rats in batch 1 who have not received B. bifidum marked signs
ofseriousinfectionaccompaniedby a contractionof the intestinal mucosa(intestinal atrophy), probably due to contamination of
E. coli. These results have disappeared (batch 3), and after treatment with bifidobacteria or did not even appear (batch 2) in rats
that received B. bifidum.