Mykyta V Sokolov, Roberto Maass-Moreno and Ronald D Neumann
National Institutes of Health, USA
Posters & Accepted Abstracts: J Stem Cell Res Ther
The molecular mechanisms and pathways elicited by Ultra-Low Doses of Ionizing Radiation (ULDIR) exposures in humans are still far from being comprehensively understood, partly due to the lack of appropriate sensitive model systems. We developed a novel, human Embryonic Stem Cell (hESC) culture model to examine such ULDIR effects in human cells. The discrepancies and controversies inherent to current state of ULDIR radiobiology is in a marked contrast to urgent socioeconomical needs to predict health risks emerging from diagnostic radiation in clinic, natural background radiation, and environmental radiological accident exposures. In our present study, we aimed to examine the whole genome gene expression alterations in a panel of hESC lines after ULDIR of 0.01 Gy of X-rays CT scan exposures. The responses of hESCs to high 1 Gy dose of radiation served as a positive control. We employed systems biology approaches, such as DNA microarrays, to examine whole genome gene expression changes in hESC lines; and, we elucidate the involvement of some well-known radiationresponsive set of genes, such as CDKN1A, GDF15, GADD45A, etc. in more detailed studies in hESCs. The results of our analysis will be presented and discussed.