Journal of Blood Disorders & Transfusion

Serum prohepcidin and iron metabolism in chloramphenicol induced anaemia in rats

3^rd International Conference on Hematology & Blood Disorders

November 02-04, 2015 Atlanta, USA

Christopher Chijindu Osubor and Ngozi Genevieve Idemili

University of Benin, Nigeria

Posters-Accepted Abstracts: J Blood Disord Transfus

Abstract:

Prohepcidin is a precursor of hepcidin; a peptide hormone synthesized in the liver and considered to be the master regulator of iron metabolism. Chloramphenicol, a bacteriostatic antimicrobial has a broad spectrum of activity and known to cause reversible bone marrow suppression, aplastic anemia amongst others. The study is aimed at investigating the regulatory mechanism of hepcidin in anemia induced by chloramphenicol in Wistar rats. The intervention of Telfairia occidentalis on anemia will also be assessed. A total of 60 rats were used (140-160 g) and divided into two groups (control group, n=30 and chloramphenicol group, n=30). Each group was further sub-divided into three groups (n=10). Serum prohepcidin and interleukin-6 (IL-6) were measured by an enzyme linked immunosorbent assay (ELISA) technique. Serum iron, ferritin and total iron binding capacity (TIBC) were determined using standard methods. Histological examinations of spleen and duodenum were carried out using Perl�??s Prussian blue (iron stain) and haematoxylin and eosin stain (cell morphology). Results show that levels of serum prohepcidin, IL-6, iron, ferritin, haemoglobin and haematocrit were significantly decreased (P<0.05) in chloramphenicol administered rats. Conversely, the total iron binding capacity was significantly increased (P<0.05) by chloramphenicol treatment when compared to control. The architecture of the histological examined organs spleen and duodenum were distorted by chloramphenicol administration. Treatment with Telfairia occidentalis greatly enhanced the haemoglobin and haematocrit parameters as well as the iron metabolism indicators. Humoral immunity of the rat models also received a boost with T. occidentalis ingestion. The implication of decreased levels of prohepcidin and IL-6 in iron deficiency anemia by chloramphenicol and the role of T. occidentalis is discussed.

Biography :

Email: ccosubor@uniben.edu