Journal of Bacteriology & Parasitology

Salmonella in some seafoods in Alexandria markets

4^th International Congress on Bacteriology and Infectious Diseases

May 16-18, 2016 San Antonio, USA

Hoda Elshamy and Wafaa Bakr

Pharos University in Alexandria, Egypt
Alexandria University, Egypt

Posters & Accepted Abstracts: J Bacteriol Parasitol

Abstract:

Food borne diseases are the most wide spread health problem in the world and they have implication both on health and development. Salmonella spp., are significant microbial hazards in seafood and rapid detection of Salmonella is an important criterion in quality control of seafood. This study aimed to determine the percentage of Salmonella in seafood from some markets in Alexandria and to study the validity of CHRO Magar Salmonella Plus medium versus conventional method; Xylose Lysine Desoxycholate (XLD) and Salmonella-Shigella (SS) agar for the isolation and identification of Salmonella in some sea foods. This study included 225 seafood samples that were obtained from local markets in different districts of Alexandria. The 225 seafood samples included shrimp, gandofli and River mussel (om-khloul) 75 samples each. Each sample was cultured after enrichment in Rappaport-Vassiliadis broth and tetrathionate broth for the detection of Salmonella on each of CHRO Magar Salmonella Plus medium versus the conventional culture media (XLD and SS agar). Suspected colonies were detected and confirmed both biochemically and serologically. A total of 22 seafood samples (9.8%) were positive for Salmonella by using the three different plating media. Its presence reflects the prevalence of the organism in sea food or the bad hygienic quality of seafood. CHRO Magar Salmonella Plus medium plated with samples previously enriched in Rappaport-Vassiliadis broth showed the highest senitivity and specificity of 95.45% and 100% respectively. The accuracy of SS agar after enrichment in TT was 42.2% and increased to 92.4% after enrichment in RV. CHRO Magar Salmonella Plus medium was found to be more valuable than XLD and SS in the detection of Salmonella from seafood samples. It provides a time saving method for the detection and presumptive identification of Salmonella from sea food specimens. Interpretation of colors was easy and all colonies of Salmonella tested displayed the same color and morphology.

Biography :

Email: hoda.elshamy@pua.edu.eg