Journal of Microbial & Biochemical Technology

Role of Pseudomonas fluorescens EGD-AQ6 biofilms in degrading elevated levels of p-hydroxybenzoate

3^rd World Congress and Expo on Applied Microbiology

November 07-09, 2016 Dubai, UAE

Saheli Ghosh, Asifa Qureshi and H J Purohit

CSIR-National Environmental Engineering Research Institute, India

Posters & Accepted Abstracts: J Microb Biochem Technol

Abstract:

Pseudomonas fluorescens EGD-AQ6 is an environmental bacterium isolated from domestic sewage waste water, India, degrades and produces flocculent, non-mucoidal biofilms under high concentration (>10 mM) of aromatic compounds like 4-hydroxybenzoate (4-HBA). The study aimed to characterize and understand the role of biofilms formed by the bacteria in combating such stress. Batch degradation experiments, fluorescent microscopy and gene expression analysis revealed its degradation efficacy under biofilm conditions. The characterization of the whole genome sequence (WGS) of the strain Pseudomonas fluorescens EGD-AQ6 showed presence of genes degrading 4-HBA like 4-hydroxybenzoate hydroxylase which degraded the compound via and three biofilm forming operons in the genome which was analyzed through RAST. One of the operon was characterized as pgaABCD locus, which encodes an adhesin, composed of polyβ(1-6) galactosamine (PGA), responsible for cell to surface and cell to cell adhesion. While pelABCDEF and alginates locus synthesizes pel and alginate polysaccharides which are composed of glucose and N-acetyl glucosamine (NAG), for building and development of extracellular polymeric matrix. Consequently these features endorse this strain to be unique in degradation of different xenobiotics under biofilm phenotype thereby proving an effective candidate in bioremediation technology. Pseudomonas fluorescens EGD-AQ6 has been submitted as reference sequence genome in NCBI.

Biography :

Email: saheli08@gmail.com