Journal of Bacteriology & Parasitology

Role of micro RNA-21 expression in early diagnosis and follow up of cancer of uterine cervix

9^th Biotechnology Congress

August 31-September 02, 2015 Orlando,Florida, USA

Parisa Enteghali

Islamic Azad University, Iran

Posters-Accepted Abstracts: J Biotechnol Biomater

Abstract:

Cancer of uterine cervix is one of the most common malignancies in women worldwide. Early detection of this malignancy is its greatest value, as it can be completely cured if diagnosed at early stages. Micro RNAs are small non-coding RNAs. Mir-21 has been demonstrated to be present in many types of cancer cells, and previous studies have shown that it is overexpressed in cervical cancer tissue. The purpose of this study was to identify Mir-21 in the plasma of patients with cancer of cervix, in order to find a sensitive and cost effective method for detection of cancer of cervix at early stages. Plasma samples of 32 patients and 10 healthy volunteers were collected and RNA was extracted. The presence of Mir-21 in the samples was checked by Real Time PCR. Primers were designed by stem-loop PCR technique. Presence of Mir-21 in the plasma was evaluated by qualification PCR and RT-Real Time PCR, which was used to measure expression of Mir-21 in plasma. These results provide clinical evidence that Mir-21 can be found in the plasma of patients in different stages of cancer of cervix. While it cannot be found in the plasma of healthy individuals, our study showed that in patients with cancer of cervix, Mir-21 can be detected during, and after the completion of the treatment. Our results showed that Mir-21 was expressed in SCC of cervix and the level of its expression correlated with the stage.

Biography :

Email: p_enteghali@yahoo.com