Journal of Blood Disorders & Transfusion

Role of immune staining in detecting extra-pattern and subtle lymphomatous infiltration in bone marrow biopsies of NHL patients

16^th World Hematology Congress

March 18-19, 2019 | Rome, Italy

Noha Bassiouny Hassan, Yasmin N E L Sakhawy and Gehan M Hamed

Ain Shams University Hospital, Egypt

Scientific Tracks Abstracts: Blood Disord Transfus

Abstract:

Introduction & Aim: Immunohistochemistry (IHC) enables the examination of a greater number of trephine biopsy levels and is helpful in determining additional scattered malignant cells. The aim of this study is to detect extrapattern and subtle lymphomatous infiltration in bone marrow biopsies using CD20 and CD3 immunostaining.

Patients & Methods: This study was conducted on 100 newly diagnosed Non Hodgkin Lymphoma (NHL) patients. Their bone marrow trephine biopsies were assessed on routine histology [Hematoxylin and Eosin (H & E)] and were further subjected to IHC using CD20 and CD3.

Results: Pattern of involvement by H&E was highlighted by IHC. It showed additional interstitial pattern in nine cases, parasinusoidal streaks in one case and highlighted a patchy pattern in another case with interstitial involvement on H&E. IHC also detected subtle infiltrations on additional 5.5% cases compared with histology alone. It helped in differentiating reactive (12 cases) and malignant lymphoid infiltration (33 cases).

Conclusion: CD20 and CD3 immunostaining performed routinely on bone marrow trephine biopsies has the ability to reveal extra-pattern of infiltration and improve detection of subtle lymphoid involvement. A combined procedure identifying several distinctive features in particular histotopography and IHC, provides a promising way of discriminating reactive from neoplastic lymphoid infiltrates in bone marrow trephine biopsies.

Recent Publications

1. Treetipsatit J (2013) Is immunohistochemistry mandatory in staging bone marrow trephine biopsy of patients with diffuse large B-Cell lymphoma (DLBCL)? Asian Archives of Pathology 9:79-80.

2. Chu P G, Loera S, Huang Q and Weiss L M (2006) Lineage determination of CD20-B-cell neoplasms: an immunohistochemical study. American Journal of Clinical Pathology 126:534-544.

3. West R B, Warnke R A and Natkunam Y (2002) The usefulness of immunohistochemistry in the diagnosis of follicular lymphoma in bone marrow biopsy specimens. American Journal of Clinical Pathology 117:636-643.

4. Chetty R, Echezarreta G, Comley M and Gatter K (1995) Immunohistochemistry in apparently normal bone marrow trephine specimens from patients with nodal follicular lymphoma. Journal of Clinical Pathology 48:1035-1038.

Biography :

Noha Bassiouny Hassan did her MD Clinical Pathology in Faculty of Medicine at Ain Shams University. She is working as a Lecturer of Clinical Pathology Hematology unit. She has a membership in the Egyptian Society of Thrombosis and Hemostasis.

E-mail: drnohabassiouny@gmail.com