Journal of Bacteriology & Parasitology

Removal of bacterial biofilm on intestinal cell surface in vitro using bacteriophage

46^th World Congress on Microbiology

September 18-19, 2017 Dublin, Ireland

Wonki Kim, Geonu Ha and Heejoon Myung

Hankuk University of Foreign Studies, South Korea

Posters & Accepted Abstracts: J Bacteriol Parasitol

Abstract:

Biofilm is a bacterial aggregate containing bacteria and secreted substances formed through quorum sensing. Bacteria inside a biofilm are much more resistant to antibiotics. Biofilm formed on the surfaces of human intestine causes various diseases. Escherichia coli O157:H7 produces shiga toxin and makes biofilm causing food poisoning. We induced formation of biofilm of these bacteria on human intestinal cell lines CT-26 and SNU-C4 in-vitro. Three phages infecting E. coli O157:H7 were selected and used to make a phage cocktail for controlling the bacterial infection. After phage treatment, remaining bacteria were counted and the culture was observed under microscope using immunostaining. The cocktail was effective in removing bacterial biofilm on surfaces of the cultured cells. The removal was in both time and dose-dependent fashion.

Biography :

Wonki Kim is a graduate student in Hankuk University of Foreign Studies, Korea. He is working on the use of bacteriophages for therapeutic purposes and he is conducting host range expansion of bacteriophages.